March 2014: This Month in JoVE - Food-Related Motivation, Pathogens in the Deli, Diagnosing ALS, and Bioengineering Cells into Patterns

Summary

Here's a look at what's coming up in the March 2014 issue of JoVE.

Protocol

Study of Phagolysosome Biogenesis in Live Macrophages

Marc Bronietzki¹, Bahram Kasmapour¹, Maximiliano Gabriel Gutierrez^1,2

¹Research Group Phagosome Biology, Helmholtz Centre for Infection Research, ²Division of Mycobacterial Research, National Institute for Medical Research

Studying Food Reward and Motivation in Humans

Hisham Ziauddeen^1,2,3, Naresh Subramaniam¹, Victoria C. Cambridge⁴, Nenad Medic^1,2, Ismaa Sadaf Farooqi², Paul C. Fletcher^1,2,3

¹Department of Psychiatry, University of Cambridge, ²Metabolic Research Laboratories, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, ³Cambridgeshire & Peterborough NHS Foundation Trust, University of Cambridge, ⁴West Anglia Comprehensive Local Research Network, Addenbrooke's Hospital

This article describes a set of methods for the measurement of food related motivation and food related goal values in humans.

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Mark A. Hughes¹, Paul M. Brennan², Andrew S. Bunting³, Mike J. Shipston¹, Alan F. Murray³

¹Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, ²Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, ³School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh

This protocol describes a microfabrication-compatible method for cell patterning on SiO₂. A predefined parylene-C design is photolithographically printed on SiO₂ wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO₂ regions.