Protocol for Drosophila ovary dissection
- Feed flies with yeast 1-2 days prior to dissecting them to fatten up the ovaries.
- Anaesthetize flies using carbon dioxide stream.
- Using a pair of tweezers, select a female fly.
- Submerge the female fly into 1X PBS.
- Grab the fly at its lower thorax with a pair of tweezers.
- Tug gently at the lower abdomen with another pair of tweezers until the internal organs in the abdomen are exposed.
- Look for the pair of ovaries and detach it from other organs (e.g. the intestines).
- Tease apart the ovarioles (if ovaries are to be used for immunostaining or in situ hybridization).
- Keep ovaries in ice-cold 1X PBS while dissecting the next fly.
Here we demonstrate a method of dissecting ovaries in Drosophila. As the method of culturing the different cell types that exist in the Drosophila ovary has yet been defined, this is a rapid method to obtain the Drosophila ovarian tissue. Essentially, we show that dissecting Drosophila ovaries involves a simple two- or three-step procedure. Subsequent treatment of these dissected ovaries depend on the downstream experiments that will be performed.
|Glass dissecting dish||Tool||Pyrex||7220-85|
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