Materials
| Name | Company | Catalog Number | Comments |
| Bugbuster | Novagen | 70584-3 | |
| COMPLETE protease inhibitor | Roche | 05 056 489 001 | |
| Glutathione magnetic beads | Pierce | 88821 | |
| Polyethylenimine, branched, average Mw ~25,000 | Sigma Aldrich | 408727-100ML | |
| OPIMEM | Life Technologies | 31985-047 | |
| Dulbecco's Modified Eagle Media | Sigma Aldrich | D5796 | |
| Fetal Bovine Serum | Life Technologies | 10270-1-6 | |
| L-Glutamine | Life Technologies | 25030-024 | |
| GFP-Trap_A | Chromotec | gta-20 | |
| GDP | Sigma Aldrich | G7127 | Highly unstable. Aliquot and store at -80 immediately upon reconstritution |
| GTPγS | Sigma Aldrich | G8634 | Highly unstable. Aliquot and store at -80 immediately upon reconstritution |
| Blocking Buffer | Sigma Aldrich | B6429 | |
| Tween-20 | Sigma Aldrich | P9416 | |
| Anti-GFP antibody | Living Colors | 632592 | Use at 1/1000 dilution |
| DyLight 800 conjugated goat anti-rabbit secondary antibody | Fisher Scientific | 10733944 | |
| Anti-PAK1 antibody | Cell Signaling | 2602S | Use at 1/1000 dilution |
| Odyssey SA Infrared Imaging System | Li-cor | 9260-11PC |
References
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- Williamson, R. C., et al. Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure. J Cell Sci. 127 (Pt 19), 4292-4307 (2014).
- Del Pozo, M. A., Price, L. S., Alderson, N. B., Ren, X. D., Schwartz, M. A. Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK. Embo J. 19 (9), 2008-2014 (2000).
- Van Rijssel, J., Hoogenboezem, M., Wester, L., Hordijk, P. L., Van Buul, J. D. The N-terminal DH-PH domain of Trio induces cell spreading and migration by regulating lamellipodia dynamics in a Rac1-dependent fashion. PLoS. 7 (1), e29912 (2012).
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