July 2014: This Month in JoVE - Shrinkage Stress in Dental Fillings, Olfactory Learning in Honeybees, Age-related Balance Changes, and Chemical Distribution in the Environment

Summary

Here's a look at what's coming up in the July 2014 issue of JoVE.

Protocol

Simultaneous Long-Term Recordings at Two Neuronal Processing Stages in Behaving Honeybees

Martin Fritz Brill, Maren Reuter, Wolfgang Rössler, Martin Fritz Strube-Bloss

Department of Behavioral Physiology and Sociobiology (Zoology II) Biozentrum, University of Würzburg

Simultaneous extracellular long term recordings from two different brain neuropiles or two different anatomical tracts were established in honey bees. These recordings allow the investigation of temporal aspects of neuronal processing across different brain areas at the single neuron as well as at the ensemble level in a behaving animal.

Shrinkage of Dental Composite in Simulated Cavity Measured with Digital Image Correlation

Jianying Li, Preetanjali Thakur, Alex S. L. Fok

Minnesota Dental Research Center for Biomaterials and Biomechanics, School of Dentistry, University of Minnesota

In order to understand the spatial development of polymerization shrinkage stress in dental resin-composite restorations, Digital Image Correlation was used to provide full-field displacement/strain measurement of restored model glass cavities by correlating images of the restoration taken before and after polymerization.

Behavioral Assessment of the Aging Mouse Vestibular System

Victoria W. K. Tung¹, Thomas J. Burton², Edward Dababneh¹, Stephanie L. Quail¹, Aaron J. Camp¹

¹Discipline of Biomedical Science, University of Sydney, ²The Bosch Institute Animal Behavioural Facility, University of Sydney

Motor control and balance performance are known to deteriorate with age. This paper presents a number of standard noninvasive behavioral tests with the addition of a simple rotary stimulus to challenge the vestibular system and show changes in balance performance in a murine model of aging.

Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

Audrey R. Matteson*¹, Denis J. Mahoney*², Travis W. Gannon², Matthew L. Polizzotto¹

¹Department of Soil Science, North Carolina State University, ²Department of Crop Science, North Carolina State University

Field lysimetry and porewater sampling allow researchers to evaluate the fate of chemicals applied to soils and established vegetation. The goal of this protocol is to demonstrate how to install required instrumentation and collect samples for chemical analysis during integrated field lysimetry and porewater sampling experiments.