Materials
Name | Company | Catalog Number | Comments |
Candida albicans ATCC 10231 Vitroids 80 CFU | Sigma-Aldrich | RQC14003-10EA | Place directly on a spread agar plate according to the manufacturer's instructions |
YPD broth | Sigma-Aldrich | Y1375 | Follow manufacturer's instructions, various providers exist |
Dextran Clinical grade MW = 200,000-300,000 | MP Biomedicals | 2101514 | Use gloves |
Heparin Sodium solution for infusion (preservative free), 1,000 IU/mL | Fannin, Wockhardt UK Ltd | FP1077 | We purchased from UCH inpatient pharmacy |
SNARF-1 Carboxylic Acid, Acetate, Succinimidyl Ester - Special Packaging | Thermo Scientific/Invitrogen | SS22801 | Use gloves |
5-(and-6)-Carboxy SNARF-1, Acetoxymethyl Ester, Acetate | Thermo Scientific/Invitrogen | C1272 | Use gloves |
Vivaglobin human IgG 160 mg/mL solution | CSL Behring | received as a gift | Various providers exist |
Normal mouse serum | Abcam | ab7486 | Various providers exist |
Lymphoprep (density gradient medium) | Alere Ltd | 1114547 | Keep sterile and away from direct sunlight, can be substituted by Ficoll-Paque |
Nigericin | Sigma-Aldrich | N7143 | Toxic, use gloves, various providers exist |
Saponin | Sigma-Aldrich | 47036 | Toxic, use gloves, various providers exist |
Dimethyl sulphoxide (DMSO) | Sigma-Aldrich | D8418 | Toxic, use gloves, various providers exist |
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) | Sigma-Aldrich | H3375 | Various providers exist |
Tris(hydroxymethyl)aminomethane (Tris) | Sigma-Aldrich | T1503 | Various providers exist |
Potassium chloride (KCl) | Sigma-Aldrich | P9333 | Various providers exist |
Sodium chloride (NaCl) | Sigma-Aldrich | S7653 | Various providers exist |
Magnesium chloride (MgCl2) | Sigma-Aldrich | M8266 | Various providers exist |
Magnesium sulphate (MgSO4) | Sigma-Aldrich | M7506 | Various providers exist |
Potassium dihydrogen phosphate (KH2PO4) | Sigma-Aldrich | P9791 | Various providers exist |
Calcium chloride (CaCl2) | Sigma-Aldrich | C1016 | Various providers exist |
Glucose | Sigma-Aldrich | D9434 | Various providers exist |
Absolute Ethanol | Sigma-Aldrich | 2860 | Various providers exist |
Diphenylene iodonium (DPI) | Sigma-Aldrich | D2926 | Various providers exist |
Zinc chloride | Sigma-Aldrich | 208086 | Various providers exist, do not dilute in phosphate based buffers as it will precipitate |
Poly L lysine | Sigma | P4707 | Various providers exist |
Laser scanning confocal microscope (Zeiss LSM 700) | Carl Zeiss | The microscope must be able to excite at 555 nm and measure emission at two channels simultaneously (560-600 nm, >610 nm) | |
Ibidi µ slide 8 well ibiTreat | Thistle Scientific | IB-80826 | Other appropriate imaging dishes can be substituted |
Soniprep 150 | MSE | Any appropriate sonicator will suffice | |
TC20 Automated Cell Counter | BioRad | Various providers exist | |
1.5 mL Protein LoBind Tubes | Sigma-Aldrich | Z666505-100EA | Specially used as Candida can stick to normal eppendorfs |
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