July 2015 - This Month in JoVE: Treating Canine Halitosis, Minimizing Workplace Stress, and Assessing Electrical Activity and Herbicide Resistance in Plants

Protocol

A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

Vicenta Salvador-Recatalà¹, W. Freddy Tjallingii²

¹Department of Plant Physiology and Biophysics, University of Würzburg, ²EPG Systems, Wageningen, The Netherlands

Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

Alessandro Di Cerbo¹, Federica Pezzuto², Sergio Canello³, Gianandrea Guidetti³, Beniamino Palmieri^1,4

¹Department of Surgery and Surgical Specialties, Azienda Ospedaliero-Universitaria Policlinico di Modena, ²Department of Clinical Microbiology, Universtity of Modena and Reggio Emilia, ³Sanypet S.p.a, ⁴AIRMO Center Milan

We describe a simple approach for diagnosis of halitosis in dogs as well as a dietary approach for its management. This protocol may be extended to the management of halitosis in humans in the near future.

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Silvia Panozzo, Laura Scarabel, Alberto Collavo, Maurizio Sattin

Institute of Agro-environmental and Forest Biology (IBAF), National Research Council (CNR), Italy

A robust and flexible approach to confirm herbicide resistance in weed populations is presented. This protocol allows the herbicide resistance levels to be inferred and applied to a wide range of weed species and herbicides with minor adaptations.

Mindfulness in Motion (MIM): An Onsite Mindfulness Based Intervention (MBI) for Chronically High Stress Work Environments to Increase Resiliency and Work Engagement

Maryanna Klatt¹, Beth Steinberg², Anne-Marie Duchemin³

¹Department of Family Medicine, The Ohio State University College of Medicine, ²Critical Care Nursing, Wexner Medical Center, ³Department of Psychiatry, Stress, Trauma, and Resilience (STAR) Program, The Ohio State University College of Medicine

The Mindfulness in Motion (MIM) protocol offers a pragmatic Mindfulness Based Intervention (MBI) on-site, for persons working in chronically high-stress work environments that significantly increases resiliency and work engagement. The protocol has proven feasible, beneficial, and is easily adaptable to other high-stress workplaces.