In JoVE Immunology & Infection, we feature a method for studying tuberculosis (TB), which affects 9 million people and causes 1.5 million deaths every year. The bacteria that cause TB spread through the air and set up infection in the lungs, and may spread to other organs. TB infection is a complex process and difficult to model in vitro. Braian et al. present a novel 3D human lung tissue model that recapitulates the dynamics that occur during TB infection, and provides a useful tool for studying this deadly infectious disease.
In JoVE Medicine, 80% of the energy used for muscle contraction is released as heat, so physical activity can increase the body's core temperature. If core temperature gets too high, it may decrease physical performance or cause heat-related disorders. So it's important to monitor core temperature during prolonged and strenuous exercise. Bongers et al. describe a telemetric temperature pill that can be swallowed so it noninvasively measures gastrointestinal temperature during exercise. This pill is useful for monitoring core temperatures in laboratory and field-based settings.
In JoVE Bioengineering, articular cartilage is particularly difficult to repair, and remains an unmet challenge for biomedical engineers. Promising approaches for regenerating cartilage include cell-based therapies. And this month, Smeriglio et al. describe a method for isolating articular chondrocytes and fabricating biomimetic hydrogels. These provide physiologically relevant microenvironments for chondrocyte expansion and maturation. The engineered cartilage tissue can then be assessed for regenerative potential.
In JoVE Neuroscience, in vitro recordings of electrophysiological activity are mostly done in two-dimensional (2D) neuronal networks. This month, Tedesco et al. present a novel 3D platform that couples neuronal networks to planar micro-electrode arrays (MEAs). Scaffolds made of glass microbeads allow neurons to form complex 3D assemblies. The greater morphological complexity allows a wider range of electrophysiological patterns compared to standard 2D networks, and more closely approximates in vivo neural networks.
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
Mariateresa Tedesco1, Monica Frega1,2, Sergio Martinoia1, Mattia Pesce3, Paolo Massobrio1
1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT)
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.
Piera Smeriglio*1, Janice H. Lai*1,2, Fan Yang1,3, Nidhi Bhutani1
1Orthopaedic Surgery Department, Stanford University, 2Mechanical Engineering Department, Stanford University, 3Bioengineering Department, Stanford University
Cartilage repair represents an unmet medical challenge and cell-based approaches to engineer human articular cartilage are a promising solution. Here, we describe three-dimensional (3D) biomimetic hydrogels as an ideal tool for the expansion and maturation of human articular chondrocytes.
Clara Braian1, Mattias Svensson2, Susanna Brighenti2, Maria Lerm1, Venkata R. Parasa1,2
1Dept. of Clinical and Experimental Medicine, Linköping University, 2Dept. of Medicine, Karolinska Institute
Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.
Using an Ingestible Telemetric Temperature Pill to Assess Gastrointestinal Temperature during Exercise
Coen C.W.G. Bongers, Maria T.E. Hopman, Thijs M.H. Eijsvogels
Radboud Institute for Health Sciences, Department of Physiology, Radboud University Medical Center
This study describes an accurate, reliable and non-invasive technique to continuously measure gastrointestinal temperature during exercise. The ingestible telemetric temperature pill is suitable to measure gastrointestinal temperature in laboratory settings as well as in field based settings.
No conflicts of interest declared.