Protocol
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Yaniv Amir, Almogit Abu-Horowitz, Ido Bachelet
Faculty of Life Sciences and the Institute for Nanotechnology & Advanced Materials, Bar-Ilan University
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here we describe a protocol for the folding of a bio-responsive robot from DNA origami, its purification and negative staining for transmission electron microscopic imaging (TEM).
An Unpredictable Chronic Mild Stress Protocol for Instigating Depressive Symptoms, Behavioral Changes and Negative Health Outcomes in Rodents
Jefferson C. Frisbee1, Steven D. Brooks1, Shyla C. Stanley1, Alexandre C. d'Audiffret2
1Department of Physiology and Pharmacology, West Virginia University Health Sciences Center, 2Division of Vascular and Endovascular Surgery, Center for Cardiovascular and Respiratory Sciences, West Virginia University Health Sciences Center
The unpredictable chronic mild stress (UCMS) protocol is a validated method for studying behavioral and physiological changes associated with chronic stress and depressive symptoms. Eight weeks of imposition of the UCMS protocol induces behavioral changes and poor health outcomes in rodents of either gender.
Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Jacqueline M. Nolting, Christine M. Szablewski, Jody L. Edwards, Sarah W. Nelson, Andrew S. Bowman
Department of Veterinary Preventive Medicine, The Ohio State University
The authors present a protocol to collect swine nasal wipes to detect and isolate influenza A viruses.
Measurement of H2S in Crude Oil and Crude Oil Headspace Using Multidimensional Gas Chromatography, Deans Switching and Sulfur-selective Detection
Nicole E. Heshka, Darcy B. Hager
CanmetENERGY, Natural Resources Canada
A multidimensional gas chromatography method for the analysis of dissolved hydrogen sulfide in liquid crude oil samples is presented. A Deans switch is used to heart-cut light sulfur gases for separation on a secondary column and detection on a sulfur chemiluminescence detector.
Disclosures
No conflicts of interest declared.
