November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

Protocol

A Method for Extracting Pigments from Squid Doryteuthis pealeii

Christopher W. DiBona¹, Thomas L. Williams¹, Sean R. Dinneen¹, Stephanie F. Jones Labadie¹, Leila F. Deravi^1,2

¹Department of Chemistry, University of New Hampshire, ²Materials Science Program, University of New Hampshire

A protocol for the extraction of pigments from the nanostructured granules in squid Doryteuthis pealeii chromatophores is presented.

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Michael Joe Vaughan^1,2, Ann Chanon³, Joshua J. Blakeslee^1,3,4

¹Center for Applied Plant Sciences, The Ohio State University, OARDC, ²Department of Plant Pathology, The Ohio State University, OARDC, ³Department of Horticulture and Crop Science, The Ohio State University, OARDC, ⁴OARDC Metabolite Analysis Cluster, The Ohio State University, OARDC

This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

Using Pharmacological Manipulation and High-precision Radio Telemetry to Study the Spatial Cognition in Free-ranging Animals

Timothy C. Roth*¹, Aaron R. Krochmal*², William B. Gerwig, IV¹, Sage Rush³, Nathaniel T. Simmons², Jeffery D. Sullivan⁴, Katrina Wachter⁵

¹Department of Psychology, Franklin and Marshall College, ²Department of Biology, Washington College, ³University of Pennsylvania, ⁴School of Forestry and Wildlife Sciences, Auburn University, ⁵Morsani College of Medicine, University of South Florida

This paper describes a novel protocol that combines the pharmacological manipulation of memory and radio telemetry to document and quantify the role of cognition in navigation.

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

Michael Staup, George Aoyagi, TeQuana Bayless, Yixin Wang, Keefe Chng

Cardiovascular and Metabolism Group, Crown Bioscience

The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primatesand assess their metabolic status from healthy to dysmetabolic.