Protocol
Self-assembling Morphologies Obtained from Helical Polycarbodiimide Copolymers and Their Triazole Derivatives
Oleg V. Kulikov, Dumindika A. Siriwardane, Gregory T. McCandless, Samsuddin F. Mahmood, Bruce M. Novak
Department of Chemistry and Biochemistry, University of Texas at Dallas
Here, we present a protocol to prepare and visualize secondary structures (e.g., fibers, toroidal architectures, and nano-spheres) derived from helical polycarbodiimides. The morphology characterized by both atomic force microscopy (AFM) and scanning electron microscopy (SEM) was shown to depend on molecular structure, concentration, and the solvent of choice.
Measuring and Altering Mating Drive in Male Drosophila melanogaster.
Christine L. Boutros*1, Lauren E. Miner*1, Ofer Mazor2,3, Stephen X. Zhang3
1F.M. Kirby Neurobiology Center, Boston Children’s Hospital, 2Harvard NeuroDiscovery Center, Harvard Medical School, 3Department of Neurobiology, Harvard Medical School
This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.
Assessing the Effects of Music Listening on Psychobiological Stress in Daily Life
Alexandra Linnemann, Jana Strahler, Urs M. Nater
Department of Psychology, Division of Clinical Biopsychology, University of Marburg
A study protocol is presented on how to assess associations between music listening and psychobiological stress (as measured by subjective stress levels, salivary cortisol, and salivary alpha-amylase) in daily life. Advice on study design, materials, methods, selection of participants, and statistical handling is provided. Representative results are presented and discussed.
Activating Autophagy by Aerobic Exercise in Mice
Altea Rocchi, Congcong He
Department of Cell and Molecular Biology, Northwestern University
Autophagy activation is beneficial in the prevention of a number of diseases. One of the physiological approaches to induce autophagy in vivo is physical exercise. Here we show how to activate autophagy by aerobic exercise and measure autophagy levels in mice.
Disclosures
No conflicts of interest declared.
