JoVE Monthly Highlights: March 2017

Protocol

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Syrena C. Fernandes, Daniel J. Wilson, Charles R. Mace

Department of Chemistry, Tufts University

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

Absolute Quantification of Aβ_1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Josef Pannee^1,2, Kaj Blennow^1,2, Henrik Zetterberg^1,2,3, Erik Portelius^1,2

¹Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, ²Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, ³UCL Institute of Neurology, Queen Square

A reference measurement procedure for the absolute quantification of Aβ_1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Laura B. Goodman¹, Renee R. Anderson¹, Marcia Slater², Elen Ortenberg², Randall W. Renshaw¹, Brittany D. Chilson¹, Melissa A. Laverack¹, John S. Beeby¹, Edward J. Dubovi¹, Amy L. Glaser¹

¹Population Medicine and Diagnostic Sciences, Cornell University Animal Health Diagnostic Center, ²Thermo Fisher Scientific Inc.

High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.

Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation

Ke Ma, Dominique P. Lippelt, Bernhard Hommel

Cognitive Psychology Unit, Leiden University

Here, we describe virtual-hand and virtual-face illusion paradigms that can be used to study body-related self-perception/-representation. They have already been used in various studies to demonstrate that, under specific conditions, a virtual hand or face can be incorporated into one's body representation, suggesting that body representations are rather flexible.