Protocol
Using Wavelet Entropy to Demonstrate how Mindfulness Practice Increases Coordination between Irregular Cerebral and Cardiac Activities
Hin Hung Sik1, Junling Gao1,2, Jicong Fan1, Bonnie Wai Yan Wu1, Hang Kin Leung1, Yeung Sam Hung2
1Centre of Buddhist Studies, The University of Hong Kong, 2Department of Electrical and Electronic Engineering, The University of Hong Kong
This manuscript describes how to use the wavelet entropy index to analyze high-density electroencephalography (EEG) and electrocardiography (ECG) data. We show that the irregularity of cerebral and cardiac activities became more coordinated during mindfulness-based stress reduction practice.
Cardiac Muscle Cell-based Actuator and Self-stabilizing Biorobot - Part 2
Neerajha Nagarajan*1, Merrel T. Holley*2, Christian Danielson2, Kidong Park2, Pinar Zorlutuna1
1Department of Aerospace and Mechanical Engineering, Bioengineering Graduate Program, University of Notre Dame, 2Division of Electrical and Computer Engineering, Louisiana State University
In this study, a biological actuator and a self-stabilizing, swimming biorobot with functionalized elastomeric cantilever arms are seeded with cardiomyocytes, cultured, and characterized for their biochemical and biomechanical properties over time.
NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Kathryn Williamson1, Emmanuel Hatzakis1,2
1Department of Food Science and Technology, The Ohio State University, 2Foods for Health Discovery Theme, The Ohio State University
Here, high-resolution 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used as a rapid and reliable tool for quantitative and qualitative analysis of encapsulated fish oil supplements.
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
Shuo Li*1,2, Sumana Raychaudhuri*1, Shigeki Watanabe1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.
Disclosures
No conflicts of interest declared.