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Biology

Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Published: August 23, 2008 doi: 10.3791/889

Summary

In this video, we demonstrate two microapplicator techniques used to infect of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.

Abstract

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

Protocol

Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.

Disclosures

The authors have nothing to disclose.

Tags

Microapplicator Infection Lepidopteran Larvae Baculovirus Protein Expression Vectors Insect Pest Control Agents Gut Infection Hemolymph Infection Polyhedra Budded Virus Baculovirus Lifecycle Insecticidal Agents Formulation Application Pest Control
Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
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Li, H., Sparks, W., Bonning, B.More

Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).

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