Guest Editor
Collection Overview
To put it simply, an organoid is a miniaturized in vitro model that phenocopies/mimics the structure and function of an organ in vivo. This simple definition belies the exquisite power that such in vitro model systems contribute to studying many aspects of the development and diseases of different organs in the body. Their utility and power have driven what can fairly be characterized as an explosive use of organoids for analyses of growth and development, disease simulation, drug screening, and other potential therapeutic approaches. To obtain necessary information, such as details of morphological structures and the dynamics of cell function and signals, it is essential to monitor organoid dynamics and cellular-subcellular responses directly and in real-time. Among different detection approaches, innovative imaging technologies support organoids' direct and quantitative observation. This Methods Collection aims to gather protocols by experts in such imaging approaches, specifically applied to stem cell biology, stem cell commitment, and lineage progression in tissue and organ regeneration.
Articles
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
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Cited by 1
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2022
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Olgu Enis Tok1,2, Gamze Demirel2,3, Yusuf Saatci1, Zeynep Akbulut3,5, Ozgecan Kayalar6, Ranan Gulhan Aktas2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University
Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
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Cited by 4
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2022
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Serena L. Cornelius1,2, Megan M. Colonnetta2,3,4,5, Katherine E. Lake1,2, Clayton A. Smith1,2, Yu-An Zhang1,2, Evanthia T. Roussos-Torres6, Sangeetha M. Reddy1,2, Elizabeth H. Chen2,3,4,5, Isaac S. Chan1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California
Advancements in Bovine Organoid Technology Using Small and Large Intestinal Monolayer Interfaces
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Cited by 2
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2024
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Generation of Functional Endodermal Hepatic Organoids
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Cited by 1
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2025
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Soheil Akbari*1, Nevin Ersoy*1,2,3, Alper Bagriyanik1,2,4, Nur Arslan1,5, Tamer Tevfik Önder6, Esra Erdal1,7
1Izmir Biomedicine and Genome Center, 2Department of Histology and Embryology, Health Science Institute, Dokuz Eylul University, 3Department of Histology and Embryology, Faculty of Medicine, Izmir Tinaztepe University, 4Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylul University, 5Department of Pediatric Gastroenterology and Metabolism, Faculty of Medicine, Dokuz Eylul University, 6Department of Medical Biology, Faculty of Medicine, Koc University, 7Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University
Image-guided In Vivo Tracking of Transplanted Distal Lung Epithelial Progenitor Cells for Pulmonary Fibrosis Using Magnetic Particle Imaging
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Cited by 1
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2025
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Saumya Nigam*1,2, Katie Uhl*3, Manvir Bamrah1,4,5, Chris Lin6, Tara E. Jager7, Cameron Lawson7, Reda E. Girgis7, Elizabeth Kenyon1,2, Jinxing Li8,9, Xiaopeng Li*3, Ping Wang*1,2
1Precision Health Program, Michigan State University, 2Department of Radiology, College of Human Medicine, Michigan State University, 3Department of Pediatrics and Human Development, Michigan State University, 4Lyman Briggs College, Michigan State University, 5College of Social Science, Michigan State University, 6Department of Chemistry, Hunter College, 7Corewell Health Medical Group, 8Institute for Quantitative Health Science and Engineering (IQ), Michigan State University, 9Department of Biomedical Engineering, College of Engineering, Michigan State University
Abstracts
Generating epithelial mammary organoids from normal and tumor tissue without passaging
Isaac Chan1,
Serena Gibbs*1
1UT Southwestern
Fast and reproducible protocol to generate functional endodermal hepatic organoids (eHEPO)
Esra Erdal*1,
Nur Arslan2,
Nevin Ersoy3,
Soheil Akbari4,
Alper Bagriyanik3
1Izmir Biomedicine and Genome Center, Izmir, Turkiye; Dokuz Eylul University, Faculty of Medicine, Department of Medical Biology, Izmir, Turkiye,
2Izmir Biomedicine and Genome Center, Izmir, Turkiye; Dokuz Eylul University, Faculty of Medicine, Department of Pediatric Gastroenterology and Metabolism, Izmir, Turkiye,
3Izmir Biomedicine and Genome Center, Izmir, Turkiye; Dokuz Eylul University, Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkiye,
4Izmir Biomedicine and Genome Center, Izmir, Turkiye
Use of epigenetic cues and mechanical stimuli to generate blastocyst-like structures from skin dermal fibroblasts
Sergio Ledda1,
Sharon Arcuri1,
Fulvio Gandolfi1,
Teresina De Iorio1,
Georgia Pennarossa*1,
Tiziana A.L. Brevini1
1Laboratory of Biomedical Embryology, Milan ITALY
A novel multi-purpose cell culture device and complementing methodologies to prevent damage and loss of organoids and spheroids and examine them in the native environment
Ranan Aktas*1
1Cellorama, USA & Cancer and Stem Cell Research Center, Istanbul, Turkey
Establishment, maintenance, differentiation, genetic manipulation and transplantation of mouse and human lacrimal gland organoids
Hans Clevers*1,
Marie Bannier-Hélaouët1
1Hubrecht Institute, KNAW, Oncode Institute
Efficient Long-Term Expansion and Differentiation of Primary Alveolar Epithelial Cells
Juan Hou1,
Victor Ho1,
Wing Chang1,
Tyler Brown1,
Uriel Peña1,
Marisa Tellis1,
Allen C. Eaves1,
Philipp Kramer*1,
Arisa Yoshikawa1,
David Rowbotham1,
Sharon A. Louis1,
Catherine Bowden1,
Natalie Ronaghan1
1STEMCELL Technologies Inc.
Canine intestinal organoids and their integration into a microfluidic gut-on-a-chip system
Minae Kawasaki1,
Yoko Ambrosini*1
1Washington State University