Expression Platform
Expression Platform - Related Videos
13:14
Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
0 Views •
Cited by 3 •
2009
Gene microarrays are powerful tools in gene expression profiling at a genome-wide level. This technology has application in a variety of biological disciplines including developmental biology and toxicology. In this video, we detail a protocol for global gene expression analysis using a comprehensive oligonucleotide microarray platform for the zebrafish.
09:26
High-throughput Protein Expression Generator Using a Microfluidic Platform
0 Views •
Cited by 22 •
2012
We present a microfluidic approach for the expression of protein arrays. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for experimental use.
07:09
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
0 Views •
Cited by 17 •
2012
In the post-human genomics era, the availability of recombinant proteins in native conformations is crucial to structural, functional and therapeutic research and development. Here, we describe a test- and large-scale protein expression system in human embryonic kidney 293T cells that can be used to produce a variety of recombinant proteins.
09:13
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
0 Views •
Cited by 1 •
2016
We developed a protocol that is fast, sensitive and reproducible for pathogen gene expression profiling during an infection.
10:37
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
0 Views •
Cited by 6 •
2016
We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.
FAQs
Regulatory elements place the selected gene sequence under control signals that the host can recognize. This compatibility allows the host’s transcription machinery to make the corresponding RNA and its translation machinery to produce a protein when the target is a protein product. Choosing suitable regulatory control connects sequence design with controlled production rather than relying on the sequence alone.
These components influence whether production is compatible with the host and whether the product can be generated efficiently. A vector carries the relevant expression arrangement, a promoter helps initiate transcription, and the host strain supplies the cellular machinery. Their coordinated selection can affect yield and quality, making component choice a central design decision.
Comparison centers on product requirements rather than yield alone. Researchers can weigh production speed, scalability, cellular processing, and post-translational modification capabilities, then match those features to the intended protein or RNA product. This framework helps explain why no single platform is universally optimal and why platform selection is part of experimental design.
Setup begins by selecting the target sequence, placing it under regulatory elements compatible with the intended host, and choosing an appropriate vector and host strain. Researchers then establish culture conditions and assess the resulting product. This sequence of decisions links molecular design to measurable production, while leaving room to optimize yield and quality.
Production can be influenced by the choice of promoter, vector, host strain, and culture parameters. These variables can be adjusted to improve yield or quality, but the best combination depends on the selected gene product and the capabilities of the host. Systematic optimization helps align cellular production with the desired experimental outcome.
They support recombinant protein production, functional studies, assay development, vaccine research, and biopharmaceutical manufacturing. In biology, the same general strategy can generate a product for studying function or for building an assay; in applied settings, it can support vaccine-related research and manufacturing workflows. The relevant platform is chosen according to product and process needs.