1. Introduction of topic/research question
2. Key variables
3. Research hypotheses
4. Defining the variables
5. Establishing conditions

Table 1. Factorial Design. Shown are the possible combinations of factors for a 2 x 2 design.
6. Measuring the dependent variable (accuracy in decoding nonverbal communication)
7. Conducting the study
Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University
A factorial design is a common type of experiment w…
1. Introduction of topic/research question
2. Key variables
3. Research hypotheses
4. Defining the variables
5. Establishing conditions

Table 1. Factorial Design. Shown are the possible combinations of factors for a 2 x 2 design.
6. Measuring the dependent variable (accuracy in decoding nonverbal communication)
7. Conducting the study
A factorial design is used when researchers need to manipulate two or more independent variables and measure the effects on a single dependent variable in the same study.
For example, if researchers wanted to know why some people are better at reading another person?s facial expressions, they would have to examine multiple factors that could influence such ability.
Rather than test many potential influences one experiment at a time, a factorial design allows the simultaneous examination of several variables within one experiment. Such design requires fewer participants, and reveals whether the various causes interact in a special way to affect the outcome.
This video demonstrates how to design and conduct a simple factorial experiment to explore how self-awareness and self-esteem may influence the ability to decipher nonverbal signals, as well as how to analyze the results and examine additional cases that use this design.
In this experiment, a two-by-two factorial design is used, consisting of two independent variables?self-awareness and self-esteem?with two levels, high and low.
To manipulate self-awareness?how conscious an individual is about their own thoughts and feelings?participants complete a geography quiz in front of a mirror in the high self-awareness group, or in the absence of a mirror for the low self-awareness group.
To simultaneously manipulate self-esteem?a person?s positive or negative evaluation of who they are as a person?participants are provided with false-feedback on the geography quiz.
Those in the high self-esteem group are told that they scored in the top 10%, with superior and above average performance, while those in the low self-esteem group learn that they scored in the bottom 50%, performing inferior and below average.
Thus, note that participants are subjected to one of four possible combinations: high self-esteem/high self-awareness; low self-esteem/high self-awareness; high self-esteem/low self-awareness; or low self-esteem/low self-awareness.
After receiving feedback, participants are asked to view numerous sets of eyes and identify the proper emotion being expressed. In this case, the dependent variable is the accuracy of decoding the nonverbal communication.
Because of the design complexity, several hypotheses are generated. The main effect hypotheses?those that focus on the effect of a single independent variable?are that those in the high levels of each condition will be more accurate judges of eye expressions than those in the low level groups.
In contrast, the interaction hypothesis?one that predicts an independent variable changes another?s influence on the dependent variable?is that the impact of self-esteem on the ability to accurately detect nonverbal communication will be enhanced for those who experience high self-awareness, but reduced for those who experience low self-awareness.
Before the participant arrives, randomly organize packets with each of the four combinations of conditions to ensure that group assignments are entirely based on chance.
To begin the experiment, meet the participant in the lab. Provide them with informed consent, a brief description of the research, sense of the procedure, the potential risks and benefits of participating, and the right to withdrawal at any time.
Depending on the assigned self-awareness condition, instruct the participant to sit in front of a one-way mirror, with blinds open and their reflection visible or closed to prevent self-reflection, to take a quiz.
Next, give each participant a sheet with 50 spaces on it and ask them to list as many countries in Europe as they can in the next 2 min.
After indicating to the participant that you are analyzing their results compared to past participants, provide feedback to them on a sheet of paper based on their randomly assigned condition.
Then, sit the participant in front of a computer to take another quiz, which asks the participant to discern facial expressions based on ambiguous eye images.
To conclude the experiment, debrief participants by telling them the nature of the study, as well as why the true purpose of the study could not be revealed beforehand.
To analyze how self-esteem and self-awareness influence the ability to decipher nonverbal expressions, average the eye interpretation quiz scores in each group and plot the means by conditions.
To determine if group differences were found, perform a two-way ANOVA to reveal any main or interaction effects. In this case, the effect on self-awareness depends on the level of self-esteem.
Contrary to the hypothesized pattern, notice that participants with high self-awareness and low self-esteem were more accurate at deciphering nonverbal expressions. However, when exposed to low self-awareness, participants were more accurate when they had high self-esteem.
Now that you are familiar with how to design and perform a two-by-two factorial experiment, let?s take a look at some other examples of this design.
In one study, potentiation of the startle reflex was measured during a low or high probability of receiving an electric shock.
Another independent variable, such as the administration of alcohol or placebo, allows for the investigation into how shock level and alcohol influence the startle response.
In another example, consider how different levels of stress could interact with the type of exercise performed. To test all of these conditions simultaneously, a two-by-two factorial design is required.
Perhaps in another situation, a researcher is interested in how students perform on an on-screen versus a written test, whereby participants? gender may influence performance. Once again, a two-by-two factorial design is necessary for simultaneous examination.
You?ve just watched JoVE?s introduction to factorial experimental design.
Now you should have a good understanding of how to design and conduct a two-by-two factorial experiment, as well as how to statistically analyze the results common to these studies. You?ve also been introduced to several examples where the use of a two-by-two factorial design is beneficial.
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Q1: What is a factorial design and why would researchers use it?
A factorial design allows researchers to manipulate two or more independent variables and measure their effects on a single dependent variable within one study. Rather than testing potential influences one experiment at a time, this approach examines several variables simultaneously, requiring fewer participants while revealing whether different causes interact to affect outcomes.
Q2: How does a 2x2 factorial design work in practice?
A 2x2 factorial design uses two independent variables, each with two levels, creating four possible condition combinations. In the nonverbal signal study, self-awareness (high/low) and self-esteem (high/low) were manipulated through a mirror and false feedback. Participants experienced one of four combinations, allowing simultaneous examination of both variables' effects on emotion decoding accuracy.
Q3: What is the difference between main effects and interaction effects in factorial designs?
Main effects focus on how a single independent variable influences the dependent variable, while interaction effects examine whether one independent variable changes another's influence on the outcome. In the study, the main effect hypothesis predicted high self-awareness and high self-esteem would improve emotion detection, whereas the interaction hypothesis predicted self-esteem's impact would depend on self-awareness levels.
Q4: How do researchers manipulate independent variables in a factorial experiment?
Researchers use specific techniques to create different levels of each independent variable. Self-awareness was manipulated by having participants complete a quiz with or without a visible mirror. Self-esteem was manipulated through false feedback, telling participants they scored in the top 10% or bottom 50% on a geography quiz, creating distinct high and low conditions.
Q5: What statistical test analyzes results from a factorial experiment?
A two-way ANOVA (analysis of variance) reveals main effects and interaction effects in factorial designs. This test determines whether group differences exist and whether the effect of one independent variable depends on the level of another. In the study, the two-way ANOVA showed that self-awareness effects on emotion detection varied based on self-esteem levels.
Q6: Why is random assignment important when organizing factorial experiment conditions?
Random assignment ensures that group assignments are based entirely on chance, eliminating selection bias and strengthening causal conclusions. Before participants arrive, researchers randomly organize packets containing each of the four condition combinations, guaranteeing that participant characteristics do not systematically influence which condition they receive.
Q7: What are some real-world applications of factorial design beyond studying nonverbal communication?
Factorial designs apply across diverse research questions. Researchers use them to examine how shock probability and alcohol administration influence startle responses, how stress levels interact with exercise types to affect outcomes, and whether test format (on-screen versus written) and gender influence student performance. This design enables simultaneous investigation of multiple factors in complex real-world scenarios.
Chapters in this video
0:00
Overview
1:10
Experimental Design
3:13
Running the Experiment
4:36
Representative Results
5:22
Applications
6:25
Summary
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