The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging.
Huang, N. F., Niiyama, H., De, A., Gambhir, S. S., Cooke, J. P. Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia. J. Vis. Exp. (23), e1034, doi:10.3791/1034 (2009).