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Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
JoVE Journal
Biology
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JoVE Journal Biology
Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
DOI:

05:29 min

February 11, 2010

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Chapters

  • 00:00Title
  • 00:37Introduction
  • 01:27Hemolymph Extraction from Euprymna scolopes
  • 02:43Hemocyte Preparation and Staining
  • 04:23Visualization of Hemocytes and Associated Bacteria
  • 04:56Conclusion

Summary

Automatic Translation

This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.

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