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Immunology and Infection
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Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

DOI:
10.3791/52412-v

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12:40 min

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April 06, 2015

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, , , , , , ,
1Institute of Immunology and Infection Research,University of Edinburgh, 2Manchester Collaborative Centre for Inflammation Research

Chapters

  • 00:05Title
  • 01:49Infection of Mice by Gavage
  • 02:55Collection of Adult H. polygyrus Worms
  • 06:06Propagation of H. polygyrus Life Cycle
  • 07:22Setting Up Cultures for HES
  • 07:57Preparation of HES
  • 10:24Results: The H. polygyrus Life Cycle Can Be Maintained Long Term and Their Excretory-secretory Products Collected
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

Heligmosomoides polygyrus is a murine nematode with powerful immunomodulatory capabilities that closely resemble those of highly-prevalent human helminth infection. Here we describe a protocol for the long-term maintenance of the H. polygyrus lifecycle.

Tags

Heligmosomoides PolygyrusNematospiroides DubiusH. BakeriGastrointestinal HelminthImmunomodulatoryChronic InfectionMiceHelminth-derived Immune RegulationAllergyAutoimmunityL3 LarvaeAdult ParasitesExcretory-secretory ProductsHES

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