A subscription to JoVE is required to view this content. Sign in or start your free trial.
16,066 Views
•
07:35 min
•
November 15, 2012
DOI:
Summary
Automatically generated
Insect hemocytes carry out many important functions, both immune and non-immune, throughout all stages of insect development. Our present knowledge of hemocyte types and function comes from studies on insect genetic models. Here, we present a method for extracting, quantifying and visualizing hemocytes from wild caterpillars.
Explore More Videos
HemocytesWild CaterpillarsInsect Immune ResponseLymph GlandEmbryonic HemocytesLarval StagesInsect Innate Immune ResponseCell MigrationChemotaxis RegulationCell ApoptosisEmbryogenesisCell SpreadingCell AggregationNodules FormationPhagocytosisEncapsulationAntimicrobial PeptidesEffector MoleculesHemocyte MorphologyHemocyte FunctionNon-model Insect Species
Read Article
Cite this Article
Stoepler, T. M., Castillo, J. C., Lill, J. T., Eleftherianos, I. A Simple Protocol for Extracting Hemocytes from Wild Caterpillars. J. Vis. Exp. (69), e4173, doi:10.3791/4173 (2012).
Copy