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Journal
/
Biochemistry
/
Identifying G-Quadruplexes in a DNA Template Using Chemical Mapping
/
Separation of Polymerized Nucleic Acid Constructs by PAGE
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Separation of Polymerized Nucleic Acid Constructs by PAGE
Separation of Polymerized Nucleic Acid Constructs by PAGE
Identifying G-Quadruplexes in a DNA Template Using Chemical Mapping
DOI:
10.3791/200253-v
•
02:37 min
•
May 12, 2023
•
Alice Sosic
,
Cristina Dal Lago
,
Richard Göttlich
,
Barbara Gatto
1
Department of Pharmaceutical and Pharmacological Sciences
,
University of Padova
,
2
Institute of Organic Chemistry
,
Justus Liebig University Giessen
Tags
PAGE
Polymerized Nucleic Acid Constructs
Gel Electrophoresis
TBE Buffer
Denaturing Gel Loading Buffer
FAM-labeled Oligonucleotide
Bsep 1
G4 TBA
Single-stranded TBA
Double-stranded TBA
Alkylation Adduct
Stranded Cleavage
DNA Fragmentation
Tris Buffer
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