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Immunology and Infection
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Staining and Imaging to Visualize Neutrophil Extracellular Traps
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Immunofluorescensfarvning af neutrofile ekstracellulære fælder i vævsprøver
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Immunology and Infection
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JoVE Journal Immunology and Infection
Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples

Immunofluorescensfarvning af neutrofile ekstracellulære fælder i vævsprøver

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:
10.3791/200337-v

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04:01 min

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August 18, 2023

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, , , , ,
1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, 2Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, 3Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

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Keywords: ImmunofluorescenceNeutrophil Extracellular TrapsBlocking SolutionDonkey SerumPrimary AntibodiesAntibody Dilution BufferMyeloperoxidaseCitrullinated Histone H3Neutrophil ElastaseSecondary AntibodiesDAPIMounting Medium

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