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Flow Cytometry Analysis of Apoptosis and the Cell Cycle in Salidroside Treated MCF-7 Cells (Video) | JoVE

Salidroside's Mechanism in Suppressing MCF-7 Cell Migration and Proliferation

Article DOI: 10.3791/200539-v • 01:00 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1, 1, 1, 1, 1, 2
1Pathology Department, Suining Central Hospital, 2General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

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Salidroside ، العمل الدوائي ، الآلية الجزيئية ، تثبيط تكاثر خلايا MCF-7 ، تثبيط هجرة الخلايا MCF-7 ، مضاد للسرطان ، مضاد لنقص الأكسجين ، مضاد للالتهابات ، مسار PI3K-AKT-HIF-1Î ±-FoxO1 ، مقايسة CCK-8 ، فحص خدش الخلية ، مقايسة الهجرة ، مقايسة غزو Matrigel ، مقايسة موت الخلايا المبرمج ، فحص دورة الخلية ، أنواع الأكسجين التفاعلية (ROS) ، نشاط Ca2 + ، Na + -K + -ATPase ، نشاط Ca2 + -ATPase ، مستويات التعبير عن البروتين ، مستويات التعبير الجيني ، تحليل اللطخة الغربية ، تحليل QRT-PCR
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