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Immunfluorescensfarging og høyoppløselig konfokalmikroskopi av CRISPR/Cas9-medierte CENP-E Knockout HeLa-celler

Mouse Ocular Lens Equatorial Region Whole Mount Preparation and Confocal Imaging to Understand the Lens Structural-Developmental Relationship (Video) | JoVE

Journal (Biology)

Mouse Ocular Lens Equatorial Region Whole Mount Preparation and Confocal Imaging to Understand the Lens Structural-Developmental Relationship (Video) | JoVE

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Journal (Biology)

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

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Immunfluorescensfarging og høyoppløselig konfokalmikroskopi av CRISPR/Cas9-medierte CENP-E Knockout HeLa-celler

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

Article DOI: 10.3791/201003-v • 03:07 min • June 23rd, 2023

June 23rd, 2023 •

Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital

Tags

Biologi utgave 196 CENP-E Knockout Centromere-assosiert protein-E Kinesin Kinetochore-microtubule Capture kromosomjustering spindelmonteringskontrollpunkt HeLa-celler fenotypebasert screening kromosomfeiljustering BUB1 Mitotisk sjekkpunkt serin/treoninkinase B (BubR1) mitotiske defekter

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