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JoVE Journal Biology

CRISPR/Cas9を介した CENP-E ノックアウトHeLa細胞の染色体調製および核型解析

Genomic Transformation of the Picoeukaryote Ostreococcus tauri

Journal (Biology)

Genomic Transformation of the Picoeukaryote Ostreococcus tauri

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Journal (Biology)

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

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CRISPR/Cas9を介した CENP-E ノックアウトHeLa細胞の染色体調製および核型解析

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

Article DOI: 10.3791/201004-v • 02:19 min • June 23rd, 2023

June 23rd, 2023 •

Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital

Tags

生物学、第196号、CENP-Eノックアウト、セントロメア関連プロテインE、キネシン、動原体微小管捕捉、染色体アライメント、紡錘体アセンブリチェックポイント、HeLa細胞、表現型ベースのスクリーニング、染色体ミスアライメント、BUB1有糸分裂チェックポイントセリン/スレオニンキナーゼB(BubR1)、有糸分裂欠損症

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