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A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
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Chapters
- 00:00Title
- 01:14Introduction
- 01:47Preparing the Animal for Imaging
- 04:46Preparing the Thinned Skull Cortical Window
- 07:00Two-photon Imaging
- 08:48Injection of the HIV-1 Neurotoxin, Tat
- 11:37Conclusion
We describe a method for repeatedly visualizing murine microglia and circulating monocytes in vivo over hours, days or weeks using transcranial two-photon microscopy. We demonstrate how to prepare a thinned-skull window that allows intermittent observation of quiescent microglia that can be activated by adjacent stereotactic injection of the HIV-1 regulatory protein Tat.
Tags
Thin-skull Window TechniqueChronic Two-photon In Vivo ImagingMurine MicrogliaNeuroinflammationOpen-skull TechniqueThinned-skull ApproachMicroglial ActivationChronic ImagingImaging SessionsTwo-photon MicroscopyStable Cortical WindowGlass CoverslipTranslucentImmunologically InertCX3CR1 GFP/+ MicePial SurfaceStereotactic Brain Injury
