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Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice
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JoVE Journal Neuroscience
Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

DOI:
10.3791/62633-v

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06:33 min

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June 22, 2021

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, ,
1Department of Neurological Sciences,University of Nebraska Medical Center

Chapters

  • 00:04Introduction
  • 00:42Start of Surgery and Craniotomy
  • 02:14Viral Injections and Implantation of Cranial Window
  • 04:10Animal Habituation and Multiphoton Imaging
  • 05:01Results: Quality Assessment of the Cranial Window
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for the implantation of a chronic cranial window for the longitudinal imaging of brain cells in awake, head-restrained mice.

Tags

Keywords: Cranial WindowIn Vivo ImagingAwake MiceViral InjectionsNeural CellsNeurodevelopmental DisordersNeurodegenerative DisordersExperience-dependent PlasticityStereotaxic FrameDura MaterIntracellular MicroinjectionCyanoacrylate AdhesiveDental Cement

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