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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Article DOI: 10.3791/2103 • 08:25 min • October 8th, 2010
October 8th, 2010 •
1, 1, 1, 2, 1
1Center for Neurologic Diseases, Brigham and Woman's Hospital and Harvard Medical School, 2Department of Physiology and Neurobiology, University of Connecticut

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Summary
Automatic Translation

In utero electroporation is a valuable method for transfecting neuronal progenitor cells in vivo. Depending upon the placement of the electrodes and the developmental timepoint of electroporation, certain subsets of cortical cells can be targeted. Targeted cells can then be analyzed in vivo or in vitro for effects of genetic alteration.

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In Utero Electroporation Primary Neuronal Culture Gene Function Subset Of Cortical Neurons Neurite Outgrowth Genetic Alterations ShRNA CDNA Constructs Chemical Transfection Viral Transduction Heterogeneous Pool Of Cell Types Glutamatergic Neurons Inhibitory Neurons Glial Cells Embryonic Rodent Cortex Deep Layers Of The Cortex Superficial Layers Of The Cortex Dorsal-medial Regions Of The Cortex Ventral-lateral Regions Of The Cortex Quantitative Analysis Step-by-step Method
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