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Cattura selettiva di 5 hydroxymethylcytosine dal DNA genomico

Article DOI: 10.3791/4441-v • 06:26 min • October 5th, 2012
October 5th, 2012 •
1, 2, 2, 1
1Department of Human Genetics, Emory University School of Medicine, 2Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago

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Descritto è un processo a due fasi usando etichettatura β-glucosil (β-GT) per trasferire una azide-glucosio al 5-HMC, seguita dalla chimica click per trasferire un linker biotina per l'arricchimento facile e densità-indipendenti. Questo metodo efficace di etichettatura e specifica consente l'arricchimento di 5-HMC con fondo estremamente basso e high-throughput mappatura epigenomic tramite sequenziamento di prossima generazione.

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Genetica Numero 68 Chimica Biofisica 5 Hydroxymethylcytosine etichettatura chimico DNA genomico high-throughput sequencing
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