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JoVE Journal
Bioengineering

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3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
 

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Article doi: 10.3791/59226
May 24th, 2019

Summary May 24th, 2019

We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.

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