Abstract
The western blotting technique measures protein expression in a cell or tissue extract using antibody-based detection of a target protein. The antibodies used in a western blot can be specific to a total protein or to unique post-translational modifications of a protein, such as sites of phosphorylation, acetylation, methylation, or ubiquitination. The broad range of available total protein and modification-specific antibodies allows researchers to study cellular signaling events involved in many biological contexts and processes. Here we provide an extensive western blot troubleshooting guide based on our many years of experience, offering solutions to save you valuable time and reagents.
