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Immunology and Infection

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荧光显微镜方法测定细菌在协会的可行性与哺乳动物细胞

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Immunology and Infection

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JoVE Journal Immunology and Infection

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Please note that all translations are automatically generated. Click here for the English version.

荧光显微镜方法测定细菌在协会的可行性与哺乳动物细胞

DOI:

10.3791/50729-v

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07:23 min

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September 05, 2013

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M. Brittany Johnson, Alison K. Criss

¹Department of Microbiology, Immunology, and Cancer Biology,University of Virginia Health Sciences Center

Chapters

00:05Title
01:37Assessing Bacterial Viability: Propidium Iodide and SYTO9
02:56Assessing Bacterial Viability: Subcellular Localization
04:52Results: Human Neutrophils and Survival of N. gonorrhoeae
06:52Conclusion

Summary

Automatic Translation

中央细菌发病机理的字段是定义是否以及如何暴露于真核细胞中后微生物生存的能力。本文概述了使用，揭示内部以及与宿主细胞相关的各个细菌的生存能力荧光染料的协议。

Tags

Keywords: Fluorescence Microscopy Bacterial Viability Mammalian Cells Neisseria Gonorrhoeae SYTO9 DAPI Propidium Iodide SYTOX Green Gentamicin Protection Assay Electron Microscopy Bacterial Pathogenesis Bacterial Localization Host-pathogen Interactions

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