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Single-celle Mikroinjektion for Cell Kommunikation Analyse

Article DOI: 10.3791/50836-v • 10:00 min • February 26th, 2017
February 26th, 2017 •
*1, *1, 1, 1, 1
1Institute Oswaldo Cruz, Laboratory of Cellular Communication, Oswaldo Cruz Foundation
* These authors contributed equally

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Vi beskriver her, hvordan du udfører en enkelt-celle mikroinjektion af Lucifer Yellow at visualisere cellulær kommunikation via gap-junctions i levende celler, og give nogle nyttige tips. Vi forventer, at dette dokument vil hjælpe alle til at vurdere graden af ​​cellulære kobling skyldes funktionelle gap junctions. Alt her beskrevne kunne være principielt tilpasses til andre fluorescerende farvestoffer med molekylvægt under 1.000 Dalton.

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Cellular Biology gap junctions cellulær kommunikation connexiner mikro-injektion Lucifer gul hemichannel
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