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Super-resolution Imaging of Neuronal Dense-core Vesicles

Article DOI: 10.3791/51394-v • 09:30 min • July 2nd, 2014
July 2nd, 2014 •
1,2, 2, 3, 2,4
1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College

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Summary
Automatic Translation

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

Tags

Super-resolution Imaging Neuronal Dense-core Vesicles Fluorescence Microscopy Diffraction-limited Resolution Super-resolution Fluorescence Microscopy Photoactivated Localization Microscopy (PALM) Lateral Resolution Cellular Structure Organization Single-color PALM Studies Vesicular Structures Fixed Cultured Neurons Labeling Neurons Photoconvertible Chimeras Vesicle Cargo
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