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High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

DOI:
10.3791/56407-v

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10:30 min

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November 16, 2017

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1Roche Pharma Research and Early Development (pRED), Neuroimmunology,Roche Innovation Center Basel

Chapters

  • 00:05Title
  • 00:53Generation of Brain Free-floating Sections
  • 02:19Cell or Organelle Labeling by Immunofluorescence Staining
  • 04:27High-resolution Confocal Imaging of the Blood-brain Barrier
  • 06:21Image Processing and 3D Reconstruction of the Neurovascular Unit
  • 09:04Results: Three-dimensional Rendering of Capillaries and Intracellular Vesicles at the Blood-brain Barrier
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.

Tags

Keywords: Blood-brain BarrierConfocal Microscopy3D ReconstructionTranscytosisImmunofluorescenceFree-floating SectionsEndothelial CellsNeurovascular UnitSubcellular OrganellesQuantificationImage Analysis

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