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Journal / Biology / BEDSTE: Stregkode Enabled Sekventering af Tetrads
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BEDSTE: Stregkode Enabled Sekventering af Tetrads

Article DOI: 10.3791/51401-v • 12:59 min • May 1st, 2014
May 1st, 2014 •
1, 1, 1, 1
1Pacific Northwest Diabetes Research Institute

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Stregkode Aktiveret Sekventering af Tetrads (BEST) erstatter de manuelle processer til at isolere, forstyrre og afstand tetrader. BEST isolater tetrader ved fluorescens-aktiveret cellesortering på agarplader adskiller sporerne ved omrøring med glasperler, og bestemmer, hvilke tilfældigt opstillede kolonier blev afledt fra den samme oprindelige tetrade hjælp molekylære stregkoder.

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Genetik gær Tetrad genetik DNA-sekventering
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