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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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Bioengineering
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JoVE Journal Bioengineering
Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

DOI:
10.3791/51102-v

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11:17 min

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February 10, 2014

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, , , ,
1Department of Bioelectronics,Ecole Nationale Superieure des Mines, 2Research and Exploratory Development Division, Applied Physics Laboratory,Johns Hopkins University

Chapters

  • 00:05Title
  • 02:05Organic Electrochemical Transistor Fabrication
  • 04:46Device Assembly
  • 05:35Cell Culture
  • 06:20Measurements with OECT and Cell Integration
  • 07:50Preparatin and Introduction of Toxic Compound
  • 08:39Results: Sensing of Barrier Tissue Disruption with and OECT
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

The Organic Electrochemical Transistor is integrated with live cells and used to monitor ion flux across the gastrointestinal epithelial barrier. In this study, an increase in ion flux, related to disruption of tight junctions, induced by the presence of the calcium chelator EGTA (ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid), is measured.

Tags

Keywords: Organic Electrochemical TransistorGastrointestinal TractEGTAParacellular FluxTransepithelial ResistanceDrug DiscoveryPathogen/toxin DiagnosticsHigh-throughput Screening

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