Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

The overall goal of this procedure is to fabricate a multiplexed microneedle based sensor for transdermal electrochemical detection of various target lytes that can be simultaneously measured. The first step is to fabricate hollow microneedle arrays using mysterial lithography and create electrode array cavities using laser microm machining. Then prepare the individual carbon pastes and fill the electrode array cavities.

Next, calibrate each of the carbon pastes over a range of physiologically relevant concentrations. Ultimately, a transdermal in vivo electrochemical device is created that is capable of simultaneously measuring multiple analytes in complex physiological microenvironments for a range of biomedical applications. The main advantage of this technique of your existing methods is the ability to perform multiplex sensing in a minimally invasive manner.