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Osteoclast Afleiding van Mouse Bone Marrow
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Osteoclast Afleiding van Mouse Bone Marrow

Article DOI: 10.3791/52056 • 06:17 min • November 6th, 2014
November 6th, 2014 •
*1, *1,2, 2, 2, 1,2, 1,2, 1,2, 1, 1, 1, 1, 1, 1, 1,2, 1,2
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University
* These authors contributed equally

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Osteoclasten zijn de voornaamste botresorberende cellen in het lichaam. Het vermogen om osteoclasten te isoleren in grote aantallen heeft tot aanzienlijke vooruitgang in het inzicht van osteoclast biologie. In dit protocol beschrijven we een werkwijze voor het isoleren, kweken en kwantificeren van osteoclasten in vitro.

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Cellular Biology osteoclasten RANKL cultuur resorptie assay botomvorming bot turnover skelet homeostase
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