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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

DOI:
10.3791/53921-v

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10:05 min

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April 07, 2016

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, , ,
1Molecular Genetics,Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy,Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH

Chapters

  • 00:05Title
  • 01:17Collecting Fertilized Eggs
  • 02:27Microinjecting Developing Embryos
  • 04:21Mounting Developing Embryos for Live Imaging
  • 06:59Compensating for Focal Drift
  • 08:04Results: Normal and Disturbed Kidney Development
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

Tags

ZebrafishKidney DevelopmentTime-lapse ImagingDissecting MicroscopeOptical SectioningDevelopmental BiologyFluorescent MicroscopyAffordable TechniqueModel OrganismsTransgenic GFPEmbryoMicro-injectionMorpholinoMicro-manipulator

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