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Immunology and Infection

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JoVE Journal Immunology and Infection
Transformación biolística de un fluorescente Tagged gen en el oportunista por hongos patógenos
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Transformación biolística de un fluorescente Tagged gen en el oportunista por hongos patógenos

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Transformación biolística es un método utilizado para generar la integración estable de ADN en el genoma de los oportunistas neoformans patógeno Cryptococcus través de la recombinación homóloga. Vamos a demostrar la transformación biolística de una construcción, que tiene el gen que codifica acetato quinasa fusionado a la mCherry etiqueta fluorescente en C. neoformans.

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Biología Molecular Número 97 la interrupción de genes, El suministro biolístico acetato quinasa la superposición de PCR la fluorescencia
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