Isolation and Culture of Hippocampal Neurons from Prenatal Mice

The overall goal of this procedure is to isolate and culture. Highly purified hippocampal neurons from prenatal mice. This is accomplished by first isolating and dissecting the hippocampus from embryonic mouse tissue.

The second step of the procedure is to dissociate individual cells by mechanical force, followed by trypsin, ization, and careful tation of the brain tissue. Finally, the dissociated cells are counted and cultured using neuro basal media and B 27 supplement. Ultimately purified hippocampal neurons in culture can be used with various molecular techniques such as immunofluorescence.