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Isolation and Culture of Hippocampal Neurons from Prenatal Mice

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

DOI:

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10:27 min

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July 26, 2012

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Michael L. Seibenhener, Marie W. Wooten

¹Department of Biological Sciences,Auburn University

Chapters

00:05Title
01:12Set-up Prior to Harvest
02:09Tissue Harvest
04:03Tissue Dissociation
05:23Neuron Trituration
06:42Cell Plating
08:43Immunofluorescence Results with Hippocampal Neuronal Cultures
09:58Conclusion

Summary

Automatic Translation

We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.

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