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Oppdage Anastasis i Vivo av CaspaseTracker Biosensor

Article DOI: 10.3791/54107-v • 20:16 min • February 1st, 2018
February 1st, 2018 •
1,2, 2, 3
1Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, 2School of Life Sciences, Chinese University of Hong Kong, 3Department of Neurosurgery, Johns Hopkins University School of Medicine

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Anastasis er teknisk utfordrende for å oppdage i vivo fordi cellene som tilbakeført celle død prosessen kan være morphologically utvisket fra vanlige friske celler. Her beskriver vi protokoller for å oppdage og spore celler som gjennomgår anastasis i levende dyr ved hjelp av nyutviklet i vivo CaspaseTracker biosensor systemet.

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Medisin problemet 132 Anastasis apoptose autophagy biosensor caspase CaspaseTracker nekrose necroptosis programmert celledød tilbakeføring av apoptose
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