Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

M. Ang&#233;lica Bello; Yolanda Ruiz-Le&#243;n; J. Vladimir Sandoval-Sierra; Svetlana Rezinciuc; Javier Di&#233;guez-Uribeondo

doi:10.3791/55031

Journal

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Biology

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مسح بروتوكولات المجهر الإلكتروني الماسح (SEM) لإشكالية النبات، طلائعيات بيضية، وعينات الفطرية

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

JoVE Journal
Biology

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JoVE Journal Biology

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

مسح بروتوكولات المجهر الإلكتروني الماسح (SEM) لإشكالية النبات، طلائعيات بيضية، وعينات الفطرية

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28,759 Views

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10:57 min

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February 03, 2017

DOI:

10.3791/55031-v

DOI:

10.3791/55031-v

•

10:57 min

February 03, 2017

•

9 Views

M. Angélica Bello¹, Yolanda Ruiz-León², J. Vladimir Sandoval-Sierra³, Svetlana Rezinciuc⁴, Javier Diéguez-Uribeondo³

¹Biodiversity and Conservation Department,Real Jardín Botánico, CSIC, ²Research Support Unit,Real Jardín Botánico, CSIC, ³Mycology Department,Real Jardín Botánico, CSIC, ⁴Division of Glycoscience, AlbaNova University Center,Royal Institute of Technology (KTH)

Transcript

Summary

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.