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Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

DOI:
10.3791/55674-v

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14:02 min

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April 21, 2017

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, , , ,
1King’s British Heart Foundation Centre,King’s College London

Chapters

  • 00:05Title
  • 01:05Extraction of Extracellular Matrix Proteins
  • 03:52Protein Precipitation
  • 04:57Sequential Deglycosylation for the Assessment of N-glycosylation Site Occupancy
  • 06:05Peptide Cleanup Using C18 Column
  • 08:32Glycopeptide Enrichment
  • 11:32Results: Glycopeptide Extraction and Analysis
  • 13:18Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes a methodology to prepare cardiovascular tissue samples for MS analysis that allows for (1) the analysis of ECM protein composition, (2) the identification of glycosylation sites, and (3) the compositional characterization of glycan forms. This methodology can be applied, with minor modifications, to the study of the ECM in other tissues.

Tags

GlycoproteomicsExtracellular MatrixGlycosylationMass SpectrometryCardiovascular DiseaseGlycopeptide AnalysisGlycosylation Site Occupancy

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