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JoVE Journal
Biochemistry

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Glycoproteomics of the Extracellular Matrix
 

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Article DOI: 10.3791/55674
April 21st, 2017

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Summary April 21st, 2017

This paper describes a methodology to prepare cardiovascular tissue samples for MS analysis that allows for (1) the analysis of ECM protein composition, (2) the identification of glycosylation sites, and (3) the compositional characterization of glycan forms. This methodology can be applied, with minor modifications, to the study of the ECM in other tissues.

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