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Serum and Plasma Copy Number Detection Using Real-time PCR
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JoVE Journal Cancer Research
Serum and Plasma Copy Number Detection Using Real-time PCR

Serum and Plasma Copy Number Detection Using Real-time PCR

DOI:
10.3791/56502-v

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09:21 min

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December 15, 2017

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, , , , ,
1Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Chapters

  • 00:05Title
  • 00:55Collection of Serum or Plasma
  • 02:20Isolating Cell-free (cfDNA) from Serum or Plasma
  • 05:15Real-time PCR and Analysis of cfDNA
  • 07:36Results: Detection of Androgen Receptor (AR) Copy Number Variations in cfDNA of Metastatic Castration-resistant Prostate Cancer (CRPC) Patients
  • 08:25Conclusion

Summary

Automatic Translation

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This manuscript describes the copy number variation analysis performed in serum or plasma DNA using real-time PCR approach. This method is suitable for the prediction of drug resistance in castration resistant prostate cancer patients, but it could be informative also for other diseases.

Tags

Keywords: SerumPlasmaCopy Number DetectionReal-time PCRCell-free DNAAndrogen ReceptorGene Copy Number VariationMetastatic Castration-resistant Prostate CancerBiomarkerLiquid BiopsyDigital PCR

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