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Chapters
- 00:04Title
- 01:01RNA Extraction
- 03:43cDNA Synthesis
- 04:55Pre-amplification
- 06:02Quantitative Real-time PCR
- 07:05Data Analysis
- 07:27Results: Use of Quantitative Real-time Reverse Transcription PCR with or without Pre-amplification to Measure the Absolute Levels of Plasma micro-RNAs
- 09:54Conclusion
This report describes a protocol for measuring the absolute levels of plasma miRNA, using quantitative real-time reverse transcription PCR with or without pre-amplification. This protocol affords better understanding of the quantity of plasma miRNAs and allows qualitative assessment of corresponding data from different studies or laboratories.
