Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Takuma Iguchi; Noriyo Niino; Satoshi Tamai; Ken Sakurai; Kazuhiko Mori

doi:10.3791/56850

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Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

JoVE Journal
Biology

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JoVE Journal Biology

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

11,561 Views

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10:23 min

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February 12, 2018

DOI:

10.3791/56850-v

DOI:

10.3791/56850-v

•

10:23 min

February 12, 2018

•

11551 Views

Takuma Iguchi¹, Noriyo Niino¹, Satoshi Tamai¹, Ken Sakurai¹, Kazuhiko Mori¹

¹Medicinal Safety Research Laboratories,Daiichi Sankyo Co., Ltd.

Summary

This report describes a protocol for measuring the absolute levels of plasma miRNA, using quantitative real-time reverse transcription PCR with or without pre-amplification. This protocol affords better understanding of the quantity of plasma miRNAs and allows qualitative assessment of corresponding data from different studies or laboratories.