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Journal / Immunology and Infection / Génération d’anticorps monoclonaux dirigés contre …
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Immunology and Infection
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Génération d’anticorps monoclonaux dirigés contre des produits naturels

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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Cet article fournit un protocole détaillé pour la préparation et l’évaluation des anticorps monoclonaux dirigés contre des produits naturels destinés à divers tests immunologiques. Cette procédure comprend l’immunisation, la fusion cellulaire, ELISA concurrent indirecte positive clone de dépistage, et la préparation de l’hybridome monoclonale. Les spécifications pour la caractérisation d’anticorps à l’aide de MALDI-TOF-MS et analyses ELISA sont également fournies.

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Immunologie et Infection numéro 146 anticorps monoclonal l’antigène artificiel produit de la nature petit composé moléculaire vaccination hybridomes
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