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Journal / Genetics / Manipulação de ploidia em Caenorhabditis elegans
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Genetics

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JoVE Journal Genetics
Manipulação de ploidia em Caenorhabditis elegans
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Manipulação de ploidia em Caenorhabditis elegans

Article DOI: 10.3791/57296 • 07:54 min • March 15th, 2018
March 15th, 2018 •
1, 1,2, 1, 1,3, 1,2,3
1Brooklyn College, Biology Department, City University of New York, 2The Graduate Center, Biology Department, City University of New York, 3Advanced Science Research Center, City University of New York

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Este método permite a geração de tetraploide e triploide nemátodos Caenorhabditis de qualquer estirpe diploide. Poliploides tensões geradas por esse método tem sido usadas para estudar interações do cromossomo em prófase meiótica, e esse método é útil para examinar questões básicas importantes no, desenvolvimento, evolução, biologia celular e câncer.

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Genética edição 133 poliploidia Caenorhabditis elegans rec-8 RNAi meiose Endoreduplicação duplicação do inteiro-genoma tamanho do genoma dose do gene biológico dimensionamento instabilidade genômica
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