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Menneskelige Pluripotent stamceller kultur på polyvinylacetat alkohol-Co-Itaconic syre Hydrogels med varierende stivhet Under Xeno-gratis forhold
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1Department of Chemical and Materials Engineering,National Central University, 2Department of Botany and Microbiology,King Saud University, 3Cathay Medical Research Institute,Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics,National Central University, 5Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, 6Department of Internal Medicine,Taiwan Landseed Hospital, 7Department of Zoology,Bharathiar University, 8Thiruvalluvar University
Chapters
- 00:04Title
- 01:00Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
- 05:02Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
- 06:37Characterization of the Expression of Pluripotency Proteins by Immunostaining
- 08:49Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
- 10:19Conclusion
En protokoll er presentert for å forberede polyvinylacetat alkohol-co-itaconic syre hydrogels med varierende stivhet, som ble podet med og uten oligopeptides, for å undersøke effekten av stivhet av biologisk materiale på differensiering og spredning stamceller. Stivhet av hydrogels ble kontrollert av crosslinking tiden.
